How To Guides


THESE GUIDES describe techniques and use of the equipment in the Polymer Analysis Laboratory. A "mirror" copy is kept in the  Applied Polymer Technology Extension Consortium (APTEC) website.






                    Analytical Ultracentrifugation




                   Materials Properties





                   Solutions  -  dn/dc

                   Thermal Analysis





LSU Office of Environmental Health and Safety (EHS)

                Online Training

                LSU University Safety Manual

                LSU's Hazardous Waste Disposal Web Page

                           Request for Hazardous Material Pickup & Disposal

                           Hazardous Waste Label form (3-18)

                Chemical Inventory Management System

                Safety Data Sheets (SDS)

                            Vermont SDS Collection

                            Link to other SDS Collections

                                                                                                                GO TO INDEX


Excel Trick:  Using Solver (two examples in the directory -- see "solver" titles)

Laboratory Notebooks (Powerpoint Presentation)(how to keep a decent lab notebook--more than you think)

Survival Skills (how to graph, program, keep records, identify resistors, and other useful stuff)

Writing (advice from the macromolecular faculty)

Data Handling (Powerpoint Presentation)       

                                                                                                                GO TO INDEX

            SOLUTIONS - dn/dc

Making Solutions

Densitometry using the Paar DMA 58 (Word)  

Differential Refractive Index (dn/dc)   Excel Spreadsheet Template

              Brice-Phoenix Manual (PDF)

              Brice-Phoenix appendix (PDF)

Abbe Zeiss refractometer

dn/dc tables and papers (Password Protected)

Solvent Properties


Nano Measurement Protocols (NIST)

                Sample Preparation Protocols (NIST)

                Physico Chemical Measurement Protocols (NIST)

                Biological Measurement Protocols (NIST)


                                                                                                        GO TO INDEX


Multiple-angle, multiple-correlator DLS (MADLS)

Guinier Plot (Radius of Gyration from Light Scattering)

Dynamic Light Scattering Minicourse

             *Final exam        *Accompanying Data File

Dynamic Light Scattering (Legacy Document)

         (Practice data for Laplace Inversion: Milk or Dow Polystyrene 1683

DLS Alignment

Bock/Siano Microemulsion recipe

Light Scattering Intensity Measurements

Zimm Plots

        Download Zip File for Program GuiDe

        GuiDe Manual; the Zimm Plot info is in an Appendix 

        Related J. Chem.Ed. article (Russo, Fong, Temyanko & Ricks, J.Chem.Ed. 1999, 76(11), 1534.

Learn about Laplace Inversion for Size Distribution (external; backup is saved on web; contact PR or RC if link is broken)

Measuring the Size of Nanoparticles in Aqueous Media Using Batch-Mode Dynamic Light Scattering

          NIST-NCL Joint Assay Protocol, PCC-1 Version 1.2 (

Malvern ZetaSizer Nano ZS


            Manuals and Tutorials (Password Protected)

                                                                                                        GO TO INDEX


Intrinsic Viscosity

Using the Wells-Brookfield Viscometer (also requires instrument manual = LVTDCP.pdf)

                                                                                                        GO TO INDEX


Overview Document

            (Practice data: Velocity and Equilibrium)

            (Step-by-step Instructions for Velocity Run)

            (Step-by-step Instructions for Equilibrium Run)

            (Video for cell assembly)

                                                                                                        GO TO INDEX



Links to Microscopy Resources (Protocols, Tutorials, Vendors, Books, etc)

Microscopy from the Very Beginning (Zeiss Copyright: see Dr. Cueto)

Photography Through the Microscope (Kodak Copyright: see Dr. Cueto)

                                                                                                        GO TO INDEX


Gel Permeation Chromatography (GPC)

Gel Permeation Chromatography with Light Scattering (GPC/LS)

            Adobe Acrobat .pdf version

            Practice Data Directory

            Wyatt Astra/Dawn Manuals, etc (Password Protected)

                                                                                                        GO TO INDEX


TA Manuals, etc (Password Protected)

                                                                                                        GO TO INDEX


Anotop filter membranes

Whatman filter information

Refractive index for various polymers

                                                                                                        GO TO INDEX



PAL Scheduling  NEW SCHEDULER (03-01-2018)

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Last Modified  02/08/2021

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