How To Guides

 

THESE GUIDES describe techniques and use of the equipment in the Polymer Analysis Laboratory. A "mirror" (modified) copy  is kept in the  Applied Polymer Technology Extension Consortium (APTEC) website.

 

 


                                INDEX

 

                    Analytical Ultracentrifugation

                    Books

                    Chromatography

                   General

                   Materials Properties

                   Microscopy

                   Safety

                   Scattering

                   Scheduling

                   Solutions  -  dn/dc

                   Thermal Analysis

                   Viscosity


                        SAFETY

 

LSU Office of Environmental Health and Safety (EHS)

                                                                                                           GO TO INDEX

 


                        GENERAL

 

Excel Tricks

            Solver Template (does a linear fit the nonlinear way).

            Solver Demo (fits sum of sine & cosine)

Laboratory Notebooks (Powerpoint Presentation) (how to keep a decent lab notebook--more than you think)

Survival Skills (how to graph, program, keep records, identify resistors, and other useful stuff)

Writing (advice from the macromolecular faculty)

Data Handling (Powerpoint Presentation)

Manuals: Link to user manuals of instruments/equipment in PAL (Password Protected)

                                                                                                                GO TO INDEX

 


                        SOLUTIONS - dn/dc

 

Making Solutions

Solvent Properties

 

Densitometry using the Paar DMA 58 (Word)

 

Differential Refractive Index (dn/dc)  

              Excel Spreadsheet Template

              Brice-Phoenix Manual (PDF)

              Brice-Phoenix appendix (PDF)

dn/dc Tutorial (Powerpoint, from APTEC)

dn/dc tables and papers (Password Protected)

 

Abbe Zeiss refractometer

 

Nano Measurement Protocols (NIST)

                Sample Preparation Protocols (NIST)

                Physico Chemical Measurement Protocols (NIST)

                Biological Measurement Protocols (NIST)

                                                                                                        GO TO INDEX

 


                        SCATTERING

 

Multiple-angle, multiple-correlator DLS (MADLS)

Guinier Plot (Radius of Gyration from Light Scattering)

Dynamic Light Scattering Minicourse

             *Final exam        *Accompanying Data File

Dynamic Light Scattering (Legacy Document)

         (Practice data for Laplace Inversion: Milk or Dow Polystyrene 1683

DLS Alignment

Bock/Siano Microemulsion recipe

Light Scattering Intensity Measurements

Zimm Plots

        Download Zip File for Program GuiDe

        GuiDe Manual; the Zimm Plot info is in an Appendix 

        Related J. Chem.Ed. article (Russo, Fong, Temyanko & Ricks, J.Chem.Ed. 1999, 76(11), 1534.

Learn about Laplace Inversion for Size Distribution (external; backup is saved on web; contact PR or RC if link is broken)

Measuring the Size of Nanoparticles in Aqueous Media Using Batch-Mode Dynamic Light Scattering

          NIST-NCL Joint Assay Protocol, PCC-1 Version 1.2 ( http://dx.doi.org/10.6028/NIST.SP.1200-6)

Malvern ZetaSizer Nano ZS

            Manuals and Tutorials (Password Protected)

                                                                                                        GO TO INDEX

 


                        VISCOSITY

 

Intrinsic Viscosity

Using the Wells-Brookfield Viscometer (also requires instrument manual = LVTDCP.pdf)

                                                                                                        GO TO INDEX

 


                        ANALYTICAL ULTRACENTRIFUGATION

 

Overview Document

            (Practice data: Velocity and Equilibrium)

            (Step-by-step Instructions for Velocity Run)

            (Step-by-step Instructions for Equilibrium Run)

            (Video for cell assembly)

                                                                                                        GO TO INDEX

 


                        MICROSCOPY

 

Links to Microscopy Resources (Protocols, Tutorials, Vendors, Books, etc)

Microscopy from the Very Beginning (Zeiss Copyright: see Dr. Cueto)

Photography Through the Microscope (Kodak Copyright: see Dr. Cueto)

                                                                                                        GO TO INDEX

 


                        CHROMATOGRAPHY

 

Gel Permeation Chromatography (GPC)

Gel Permeation Chromatography with Light Scattering (GPC/LS)

            Adobe Acrobat .pdf version

            Practice Data

            Wyatt Astra/Dawn Manuals, Tutorials, etc (Password Protected)

                                                                                                        GO TO INDEX

 


                        THERMAL ANALYSIS

 

TA Manuals, etc (Password Protected)

                                                                                                        GO TO INDEX

 


                        MATERIALS PROPERTIES

 

  Anotop (Whatman (Cytiva) filter membranes

            VWR Store

            Fishersci Store

           

Whatman Filter Information

Refractive Index of Polymers (From the Polymer Properties Database)

                                                                                                        GO TO INDEX

 


 

                        SCHEDULING

 

PAL Scheduling      NEW SCHEDULER (03-01-2018)

                                                                                                        GO TO INDEX

 


Last Modified  05/28/2021

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