How To Guides


THESE GUIDES describe techniques and use of the equipment in the Polymer Analysis Laboratory. A "mirror" (modified) copy  is kept in the  Applied Polymer Technology Extension Consortium (APTEC) website.





                    Analytical Ultracentrifugation


                    Chromatography (GPC/ SEC)


                   Materials Properties





                   Solutions  -  dn/dc

                   Thermal Analysis




LSU Office of Environmental Health and Safety (EHS)

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Excel Tricks

                Solver Template (does a linear fit the nonlinear way).

                Solver Demo (fits sum of sine & cosine)

Laboratory Notebooks (Powerpoint Presentation) (how to keep a decent lab notebook--more than you think)

Survival Skills (how to graph, program, keep records, identify resistors, and other useful stuff)

Writing (advice from the macromolecular faculty)

Data Handling (Powerpoint Presentation)

Manuals: Link to user manuals of instruments/equipment in PAL (Password Protected)

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                        SOLUTIONS - dn/dc


Making Solutions

Solvent Properties


Densitometry using the Paar DMA 58 (Word)


Differential Refractive Index (dn/dc)  

                Excel Spreadsheet Template

                Brice-Phoenix Manual (PDF)

                Brice-Phoenix appendix (PDF)

dn/dc Tutorial (Powerpoint, from APTEC)

dn/dc tables and papers (Password Protected)


Abbe Zeiss refractometer


Nano Measurement Protocols (NIST)

                Sample Preparation Protocols (NIST)

                Physico Chemical Measurement Protocols (NIST)

                Biological Measurement Protocols (NIST)

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List of BOOKS Related to Light Scattering

Multiple-angle, multiple-correlator DLS (MADLS)

Guinier Plot (Radius of Gyration from Light Scattering)

Dynamic Light Scattering Minicourse

                *Final exam        *Accompanying Data File

Dynamic Light Scattering (Legacy Document)

                (Practice data for Laplace Inversion: Milk or Dow Polystyrene 1683

DLS Alignment

Bock/Siano Microemulsion recipe

Light Scattering Intensity Measurements

Zimm Plots

                Download Zip File for Program GuiDe

                GuiDe Manual; the Zimm Plot info is in an Appendix

                Related J. Chem.Ed. article (Russo, Fong, Temyanko & Ricks, J.Chem.Ed. 1999, 76(11), 1534.

Learn about Distribution analysis for Size Distribution (External link @


Measuring the Size of Nanoparticles in Aqueous Media Using Batch-Mode Dynamic Light Scattering

          NIST-NCL Joint Assay Protocol, PCC-1 Version 1.2 (


Guide for Dynamic Light Scattering (DLS) Sample Preparation (External link @ Brookhaven Instruments)


Malvern ZetaSizer Nano ZS

                Tutorials, Manuals, etc. (Password Protected)

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Intrinsic Viscosity

Using the Wells-Brookfield Viscometer (also requires instrument manual = LVTDCP.pdf)

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Overview Document

                (Practice data: Velocity and Equilibrium)

                (Step-by-step Instructions for Velocity Run)

                (Step-by-step Instructions for Equilibrium Run)

                (Video for cell assembly)

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Links to Microscopy Resources (Protocols, Tutorials, Vendors, Books, etc)

List of BOOKS Related to Microscopy

Microscopy from the Very Beginning (Zeiss Copyright: see Dr. Cueto)

Photography Through the Microscope (Kodak Copyright: see Dr. Cueto)

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                        CHROMATOGRAPHY (GPC/SEC)



Manuals, Tutorials, Papers, Articles, etc. (Password Protected)

Gel Permeation Chromatography (GPC)

Gel Permeation Chromatography with Light Scattering (GPC/LS)

                Adobe Acrobat .pdf version

                Practice Data


            List of BOOKS Related to General Chromatography

            List of BOOKS Related to GPC (SEC)


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                        THERMAL ANALYSIS


List of BOOKS Related to Thermal Analysis

TA Manuals, Help, etc. (Password Protected)

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                        MATERIALS PROPERTIES


  Anotop (Whatman (Cytiva) filter membranes

            VWR Store

            Fishersci Store


Whatman Filter Information

Refractive Index of Polymers (From the Polymer Properties Database)

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PAL Scheduling 

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Last Modified  07/22/2021

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