How To Guides


THESE GUIDES describe techniques and use of the equipment in the Polymer Analysis Laboratory. A "mirror" (modified) copy  is kept in the  Applied Polymer Technology Extension Consortium (APTEC) website.




                      Analytical Ultracentrifugation


                    Chromatography (GPC/ SEC)


                   Materials Properties





                   Solutions  -  dn/dc


                   Thermal Analysis




LSU Office of Environmental Health and Safety (EHS)

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Excel Tricks

                Solver Template (does a linear fit the nonlinear way)(.xlsx)

                Solver Demo (fits sum of sine & cosine)(.xls)

Laboratory Notebooks (.ppt) (how to keep a decent lab notebook--more than you think)

Survival Skills (.doc) (how to graph, program, keep records, identify resistors, and other useful stuff)

Writing (advice from the macromolecular faculty)

Data Handling (.ppt)

Manuals: Link to user manuals of instruments/equipment in PAL (Password Protected)

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Making Solutions (.doc)

Solvent Properties


Densitometry using the Paar DMA 58(.doc)


Differential Refractive Index (dn/dc)(.doc)  

                Excel Spreadsheet Template(.xls)

                Brice-Phoenix Manual (.pdf)

                Brice-Phoenix appendix (.pdf)

dn/dc Tutorial (from APTEC) (.pptx)

dn/dc tables and papers (Password Protected)


Abbe Zeiss refractometer (.pdf)


Nano Measurement Protocols (NIST)

                Sample Preparation Protocols (NIST)

                Physico Chemical Measurement Protocols (NIST)

                Biological Measurement Protocols (NIST)

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List of BOOKS Related to Light Scattering

Multiple-angle, multiple-correlator DLS (MADLS) (.doc)  

Guinier Plot (Radius of Gyration from Light Scattering) (.doc)  

Dynamic Light Scattering Minicourse (.pdf)

                *Final exam (.pdf)       *Accompanying Data File (.xlsx)

Dynamic Light Scattering (Legacy Document) (.doc)

                (Practice data for Laplace Inversion: Milk or Dow Polystyrene 1683

DLS Alignment (.pdf)

Bock/Siano Microemulsion recipe (.pdf)

Light Scattering Intensity Measurements (.doc)

Zimm Plots

                Download Zip File for Program GuiDe (.zip)

                GuiDe Manual; the Zimm Plot info is in an Appendix (.doc)

                Related J. Chem.Ed. article (Russo, Fong, Temyanko & Ricks, J.Chem.Ed. 1999, 76(11), 1534.

Learn about Distribution analysis for Size Distribution (External link @


Measuring the Size of Nanoparticles in Aqueous Media Using Batch-Mode Dynamic Light Scattering (.pdf)

          NIST-NCL Joint Assay Protocol, PCC-1 Version 1.2 (


Guide for Dynamic Light Scattering (DLS) Sample Preparation (External link @ Brookhaven Instruments)


Malvern ZetaSizer Nano ZS

                Tutorials, Manuals, etc. (Password Protected)

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Intrinsic Viscosity (.doc)

Using the Wells-Brookfield Viscometer (.doc) (also requires instrument manual  (BrookfieldLVTDCP (.pdf)))

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Overview Document (.doc)

                (Practice data: Velocity  (.htm) and Equilibrium(.txt))

                (Step-by-step Instructions for Velocity Run) (.doc)

                (Step-by-step Instructions for Equilibrium Run) (.doc)

                (Video for cell assembly; JOVE, doi: 10.3791/1530)

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Links to Microscopy Resources (Protocols, Tutorials, Vendors, Books, etc)

List of BOOKS Related to Microscopy

Microscopy from the Very Beginning (Zeiss Copyright: see Dr. Cueto)

Photography Through the Microscope (Kodak Copyright: see Dr. Cueto)

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Manuals, Tutorials, Papers, Articles, etc. (Password Protected)

Gel Permeation Chromatography (GPC) (.doc)

Gel Permeation Chromatography with Light Scattering (GPC/LS) (.doc) (.pdf version)

                Practice Data (.zip)


            List of BOOKS Related to General Chromatography

            List of BOOKS Related to GPC (SEC)


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                            SPECTROSCOPY (UV-Vis / FL / IR / Raman)


Manuals, Tutorials, Papers, Articles, etc. (Password Protected)



            List of BOOKS Related to Spectroscopy                  

            List of BOOKS Related to Fluorescence

            List of BOOKS Related to Infrared and Raman


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TA Manuals, Help, etc. (Password Protected)

List of BOOKS Related to Thermal Analysis

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  Anotop (Whatman (Cytiva)) filter membranes

            VWR Store

            Fishersci Store


Whatman Filter Information  (.pdf)

Refractive Index of Polymers (From the Polymer Properties Database)

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PAL Scheduling (Please Note: New Scheduler as of 09/27/2021)

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Last Modified  01/19/2022

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