How To Guides

 

THESE GUIDES describe techniques and use of the equipment in the Polymer Analysis Laboratory. A "mirror" (modified) copy  is kept in the  Applied Polymer Technology Extension Consortium (APTEC) website.

 

 


INDEX

                      Analytical Ultracentrifugation

                    Books

                    Chromatography (GPC/ SEC)

                   General

                   Materials Properties

                   Microscopy

                   Safety

                   Scattering

                   Scheduling

                   Solutions  -  dn/dc

                   Thermal Analysis

                   Viscosity

 


SAFETY

LSU Office of Environmental Health and Safety (EHS)

                                                                                                           GO TO INDEX

 


GENERAL

Excel Tricks

                Solver Template (does a linear fit the nonlinear way)(.xlsx)

                Solver Demo (fits sum of sine & cosine)(.xls)

Laboratory Notebooks (.ppt) (how to keep a decent lab notebook--more than you think)

Survival Skills (.doc) (how to graph, program, keep records, identify resistors, and other useful stuff)

Writing (advice from the macromolecular faculty)

Data Handling (.ppt)

Manuals: Link to user manuals of instruments/equipment in PAL (Password Protected)

                                                                                                                GO TO INDEX

 


SOLUTIONS - dn/dc

Making Solutions (.doc)

Solvent Properties

 

Densitometry using the Paar DMA 58(.doc)

 

Differential Refractive Index (dn/dc)(.doc)  

                Excel Spreadsheet Template(.xls)

                Brice-Phoenix Manual (.pdf)

                Brice-Phoenix appendix (.pdf)

dn/dc Tutorial (from APTEC) (.pptx)

dn/dc tables and papers (Password Protected)

 

Abbe Zeiss refractometer (.pdf)

 

Nano Measurement Protocols (NIST)

                Sample Preparation Protocols (NIST)

                Physico Chemical Measurement Protocols (NIST)

                Biological Measurement Protocols (NIST)

                                                                                                        GO TO INDEX

 


SCATTERING

List of BOOKS Related to Light Scattering

Multiple-angle, multiple-correlator DLS (MADLS) (.doc)  

Guinier Plot (Radius of Gyration from Light Scattering) (.doc)  

Dynamic Light Scattering Minicourse (.pdf)

                *Final exam (.pdf)       *Accompanying Data File (.xlsx)

Dynamic Light Scattering (Legacy Document) (.doc)

                (Practice data for Laplace Inversion: Milk or Dow Polystyrene 1683

DLS Alignment (.pdf)

Bock/Siano Microemulsion recipe (.pdf)

Light Scattering Intensity Measurements (.doc)

Zimm Plots

                Download Zip File for Program GuiDe (.zip)

                GuiDe Manual; the Zimm Plot info is in an Appendix (.doc)

                Related J. Chem.Ed. article (Russo, Fong, Temyanko & Ricks, J.Chem.Ed. 1999, 76(11), 1534.

Learn about Distribution analysis for Size Distribution (External link @ softscientific.com)

 

Measuring the Size of Nanoparticles in Aqueous Media Using Batch-Mode Dynamic Light Scattering (.pdf)

          NIST-NCL Joint Assay Protocol, PCC-1 Version 1.2 ( http://dx.doi.org/10.6028/NIST.SP.1200-6)

 

Guide for Dynamic Light Scattering (DLS) Sample Preparation (External link @ Brookhaven Instruments)

 

Malvern ZetaSizer Nano ZS

                Tutorials, Manuals, etc. (Password Protected)

                                                                                                        GO TO INDEX

 


VISCOSITY

Intrinsic Viscosity (.doc)

Using the Wells-Brookfield Viscometer (.doc) (also requires instrument manual  (BrookfieldLVTDCP (.pdf)))

                                                                                                        GO TO INDEX

 


ANALYTICAL ULTRACENTRIFUGATION

Overview Document (.doc)

                (Practice data: Velocity  (.htm) and Equilibrium(.txt))

                (Step-by-step Instructions for Velocity Run) (.doc)

                (Step-by-step Instructions for Equilibrium Run) (.doc)

                (Video for cell assembly; JOVE, doi: 10.3791/1530)

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MICROSCOPY

Links to Microscopy Resources (Protocols, Tutorials, Vendors, Books, etc)

List of BOOKS Related to Microscopy

Microscopy from the Very Beginning (Zeiss Copyright: see Dr. Cueto)

Photography Through the Microscope (Kodak Copyright: see Dr. Cueto)

                                                                                                        GO TO INDEX

 


CHROMATOGRAPHY (GPC/SEC)

Manuals, Tutorials, Papers, Articles, etc. (Password Protected)

Gel Permeation Chromatography (GPC) (.doc)

Gel Permeation Chromatography with Light Scattering (GPC/LS) (.doc) (.pdf version)

                Practice Data (.zip)

BOOKS

            List of BOOKS Related to General Chromatography

            List of BOOKS Related to GPC (SEC)

 

                                                                                                        GO TO INDEX

 


THERMAL ANALYSIS

TA Manuals, Help, etc. (Password Protected)

List of BOOKS Related to Thermal Analysis

                                                                                                        GO TO INDEX

 


MATERIALS PROPERTIES

  Anotop (Whatman (Cytiva)) filter membranes

            VWR Store

            Fishersci Store

           

Whatman Filter Information  (.pdf)

Refractive Index of Polymers (From the Polymer Properties Database)

                                                                                                        GO TO INDEX

 


SCHEDULING

PAL Scheduling 

                                                                                                        GO TO INDEX

 


Last Modified  08/03/2021

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